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Stock Code 300487
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Agarose-Based Chromatography Resins
Agarose-Based Chromatography Resins
Affinity Chromatography – Agarose
Affinity Chromatography – Agarose
Metal Chelate (IDA, NTA)
Metal Chelate (IDA, NTA)

Seplife® 6AG NTA/90

  • Can be loaded with a range of divalent metals such as Ni, Co, Cu, Fe, Zn showing high affinity for His-tagged proteins
  • Suitable for small scale and large scale purifications of His-tagged proteins
  • High dynamic binding capacity CIP stability <1.0M NaOH
  • Seplife® 6AG NTA/90 is a metal affinity chromatography resin based on a 6% highly crossed linked agarose functionalized with nitrilotriacetic acid (NTA).
    Seplife® 6AG NTA/90 is based on highly cross-linked (6%) agarose functionalized with NTA and ready to be loaded with a divalent metal of choice for affinity binding of His-tag proteins and has a particle size in the range 45-165 micron.
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Description

Seplife® 6AG NTA/90 is a metal affinity chromatography resin based on a 6% highly crossed linked agarose functionalized with nitrilotriacetic acid (NTA).

l Seplife® 6AG NTA/90 can be loaded with a range of divalent metals such as Ni, Co, Cu, Fe, Zn showing high affinity for His-tagged proteins.

l Suitable for small scale and large scale purifications of His-tagged proteins from other proteins in the feed.

l Seplife® 6AG NTA/90 resin provides high dynamic binding capacity toward His-tag proteins upon loading with divalent metal.

l High stability to CIP (cleaning in place) up to 1.0M NaOH.

l Regulatory Support File (RSF) documentation is available for Seplife® 6AG NTA/90

Seplife® 6AG NTA/90 is based on highly cross-linked (6%) agarose functionalized with NTA and ready to be loaded with a divalent metal of choice for affinity binding of His-tag proteins and has a particle size in the range 45- 165 microns. 

Properties

Product

Seplife® 6AG NTA/90

Appearance

White spherical beads

Type

Metal affinity chromatography resin (NTA)

Matrix

6% cross-linked agarose

Ligand

Nitrilotriacetic acid

Particle size range (μm)

45-165

pH stability

3-13 (operational), 2-14 (CIP)

Chemical Stability

Stable in all common aqueous buffers; 1M sodium hydroxide; 8M urea; 6M guanidine hydrochloride; 70% ethanol.

Flow rate* (cm/h)

max 750cm/h

Shipped as

20% ethanol slurry

*Testing conditions: Chromatography column 16mm×200mm; column bed height 20cm; temperature 25°C; mobile phase water.

 

Storage

Sealed and stored at 4~30°C (preservation solution is 20% ethanol) in a ventilated, dry and clean place, do not freeze. 

Ordering information

Product Name

References

Pack Size

Seplife®6AG NTA/90

A4003022

25ml

A4003023

100ml

A4003024

500ml

A4003025

1L

A4003026

5L

A4003027

10L

Production date: See label

Expiry Date : 5 years, under proper storage conditions

 

Manufacturer: Sunresin New Materials Co. Ltd.

Add:No. 135, Jinye Rd, Xi'an Hi-tech Industrial Development Zone, Shaanxi, 710076, China

www.seplite.com     www.sunresin.com

E-mail: info.lifescience@sunresin.com

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