Seplife® DX CM 25/80

Designed for ion exchange chromatography of biomolecules
Supplied in dry form
Regulatory Support File (RSF) is available for Seplife® DX DEAE 50/80
Seplife® DX DEAE 50/80 is a hydrophilic ion exchange media with controlled pore size distribution used in industrial applications, prepared by crosslinked dextran functionalized with weak base anion groups.
Seplife® DX DEAE 50/80 is an ion exchange chromatographic resin based on crosslinked dextran functionalized with weak base anion with a large particle size (40-120 micron).

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Products

Properties

Product	Seplife® DX CM 25/80
Appearance	White spherical beads
Type	Weak acid cation - Carboxy methyl
Matrix	Crossed linked dextran
Ion exchange capacity (mmol/g)	4.0-5.0 (H⁺)
Particle size (dry, µm)	40-120
Swelling property (ml/g)	6.5-8.5
pH stability	6-10 (operational), 2-13 (CIP)
Chemical Stability	Stable in all common aqueous buffers; 1M sodium hydroxide; 8M urea; 6M guanidine hydrochloride; 70% ethanol.
Flow rate* (cm/h)	max. 600
Shipped as	Dry

*Testing conditions: Chromatography column 16mm×200mm; column bed height 15cm; temperature 25°C; mobile phase 0.1M NaCl

Instructions

Seplife^® DX CM 25/80 is provided as a dry powder and needs to be swollen when used. The swelling ratio depends on the buffer solution used. The swelling ratio can vary significantly in different solution compositions. Do not use magnetic stirring during swelling, to avoid breaking the particles.

Product pretreatment

Weigh the required amount of Seplife^® DX CM 25/80, add 50~100 times distilled water or equilibration buffer, and let it swell. Swelling typically takes 1-2 days at room temperature, or 2 hours in boiling water.

Column packing

Column packing should be done according to standard operating procedures. It is important to ensure that each material is at its working temperature, and the chromatography media may need to be degassed before column packing.

Note: If the column is packed at the maximum linear flow rate, the flow rate during the subsequent chromatographic separation should not exceed 75% of the column packing flow rate.

Equilibration

Equilibrate the column with an appropriate 2-5 column volume buffer. Ensure the conductivity and pH of the effluent are the same as the buffer.

Sample feeding

Determine the loading amount according to the target product concentration and the loading capacity of the media. Samples with particulates and precipitate should be filtered or centrifuged before the chromatography purification.

Cleaning

After loading the sample, equilibrate the column with loading buffer to wash away unbound proteins and impurities until the conductivity and pH of the effluent are exactly the same as those of the loading buffer.

Elution

Use continuous or gradient elution with increasing salt concentration in the buffer or increasing pH.

Regeneration

First remove the impurities from the column with 1-2M NaCl. Then remove the salt on the column with distilled water or equilibration buffer.

Storage

Seplife^® DX CM 25/80 dry powder should be stored in a dry, ventilated and clean place at 4-30℃; the hydrated media should be stored in 20% ethanol solution or 0.01M NaOH at 4-8℃.

Ordering information

Product Name	References	Pack Size
Seplife^® DX CM 25/80	D2027210	25g
	D2027211	100g
	D2027212	500g
	D2027213	1kg
	D2027214	5kg
	D2027215	10kg

Production date: See label

Expiry Date: 5 years, under proper storage conditions

Manufacturer: Sunresin New Materials Co. Ltd.

Add:No. 135, Jinye Rd, Xi'an Hi-tech Industrial Development Zone, Shaanxi, 710076, China

www.seplite.com www.sunresin.com

E-mail: info.lifescience@sunresin.com