Instructions

Seplife®DX DEAE 25/80 is provided as a dry powder and needs to be swollen when used. The swelling ratio depends on the buffer solution used. The swelling ratio can vary significantly in different solution compositions. Do not use magnetic stirring during swelling, to avoid breaking the particles.

Product pretreatment   

Weigh the required amount of Seplife®DX DEAE 25/80, add 50~100 times distilled water or equilibration buffer, and let it swell. Swelling typically takes 1~2 days at room temperature, or 2 hours in boiling water.

Column packing

Column packing should be done according to standard operating procedures. It is important to ensure that each material is at its working temperature, and the chromatography media may need to be degassed before column packing.

Note

If the column is packed at the maximum linear flow rate, the flow rate during the subsequent chromatographic separation should not exceed 75% of the column packing flow rate.

Equilibration

Equilibrate the column with an appropriate 2-5 column volume buffer. Ensure the conductivity and pH of the effluent are the same as the buffer.

Sample feeding

Determine the loading amount according to the target product concentration and the loading capacity of the media. Samples with particulates and precipitate should be filtered or centrifuged before the chromatography purification.

Cleaning

After loading the sample, equilibrate the column with loading buffer to wash away unbound proteins and impurities until the conductivity and pH of the effluent are exactly the same as those of the loading buffer.

Elution

Use continuous or gradient elution with increasing salt concentration in the buffer or decreasing pH.

Regeneration

First wash off the impurity proteins on the column with 1~2M NaCl. Then wash off the salt from the column with distilled water, to stabilized the conductivity and pH of the effluent. Then pass the loading buffer through the column with until the conductivity and pH of the effluent are stable.

Storage

Seplife®DX DEAE 25/80 dry powder should be stored in a dry, ventilated and clean place at 4~30℃; the hydrated media should be stored in 20% ethanol solution or 0.1M NaOH at 4~8℃.

Ordering information

Production date: See label

Expiry date: 5 years, under proper storage conditions

Manufacturer: Sunresin New Materials Co. Ltd.

Add:No. 135, Jinye Rd, Xi'an 

Hi-Tech Industrial Development Zone, Shaanxi, 710076, China

www.seplite.com    www.sunresin.com

E-mail: info.lifescience@sunresin.com